An injectable thermosensitive polymeric hydrogel for sustained release of Avastin® to treat posterior segment disease.

摘要: Delivery of drugs, especially bioactive macromolecules such as proteins and nucleic acids, to the posterior segment is still a significant challenge for pharmaceutical scientists. In present study, we developed an injectable thermosensitive polymeric hydrogel sustained release Avastin(®) treat disorders. The payload poly(lactic acid-co-glycolic acid)-poly(ethylene glycol)-poly(lactic acid) (PLGA-PEG-PLGA) did not influence its inherent sol-gel transition behavior, but shifted lower temperature. resulting Avastin(®)/PLGA-PEG-PLGA hydrogels had porous structure (pore size, 100 ∼ 150 μm) determined by scanning electron microcopy (SEM), facilitating over period up 14 days in vitro. PLGA-PEG-PLGA was immediately formed vitreous humor after intravitreal injection, followed slow clearance 8 week study period. exhibited no apparent toxicity against retinal tissue, indicated absence inflammation, necrosis, stress responses, using optical coherence tomography (OCT) histological/immunochemical analyses. Electrophysiology (ERG) examination also showed that affect function. vivo pharmacokinetic studies use greatly extended time retina injection. Together, these results demonstrated promising candidate ocular drug delivery Avastin(®)via