Bladder acellular matrix graft in rats: Its neurophysiologic properties and mRNA expression of growth factors TGF‐α and TGF‐β

摘要: To assess the neurophysiologic properties and molecular mechanisms of bladder acellular matrix graft (BAMG), we performed cystometric studies in male Sprague-Dawley rats (n = 46) at varying intervals. The animals were assigned to 3 groups: 1) normal, 2) partial cystectomy (>50%), 3) (>50%) grafting with a BAMG equal size. Additionally, matrix-grafted host bladders processed for analysis mRNA expression transforming growth factor (TGF)-alpha, TGF-beta1, TGF-beta2, TGF-beta3 by reverse transcriptase polymerase chain reaction. Matrix-grafted showed significantly higher capacity 6 weeks 4 months than those alone, normal controls (P < or 0.01). Residual urine volume was increased months. Electrostimulation pelvic nerve provoked generalized contractions, response that reduced atropine hexamethonium. Variable induction TGF-alpha, gene transcription evident BAMG, prominent TGF-alpha TGF-beta1 after surgery. These results detrusor responses stimulation provide further evidence components do not interfere components. rat generate, although over time, adequate intravesical pressure produce sustained voiding. Gene different factors may be significant understanding their role development differentiation replacement.