De novo cartilage generation using calcium alginate-chondrocyte constructs.

摘要: These studies investigated the utility of calcium alginate as a biocompatible polymer matrix within which large numbers chondrocytes could be held successfully in three-dimensional structure and implanted. Further, ability chondrocyte-calcium constructs to engraft generate new cartilage was examined. Chondrocytes isolated from calf shoulders were mixed with 1.5% sodium solution cell suspensions densities 0, 1.0, 5.0, 10.0 X 10 6 chondrocytes/ml. The gelled create disks that placed subcutaneous pockets on dorsums nude mice. concentration CaCl 2 used make also varied. A total 20 mice implanted 67 bovine constructs. Samples an initial cellular density at least 5.0 chondrocytes/ml demonstrated gross formation 12 weeks after implantation. Cartilage observed microscopically specimens low 1.0 histoarchitecture closely resembled native cartilage. independent (15 100 mM) or (0.5% 4.0%) gel polymerization.