Construction and use of a dominant, selectable marker: a Harvey sarcoma virus-dihydrofolate reductase chimera.

摘要: The transcriptional promoter of the Harvey sarcoma virus long terminal repeat has been used to construct a biologically active dihydrofolate reductase chimera. construction placed at 5' end cDNA. This chimera mediated methotrexate resistance when introduced into wild-type NIH3T3 mouse cells by transfection. chimeric sequences were expressed in form polyadenylated RNA and protein amplified methotrexate-resistant transfectants selected grow increasing concentrations. was dominant acting able confer phenotype on cells. It cotransfection experiments with DNA from human tumor obtain foci transformed resulting uptake exogenous DNA. transfected carried double minute chromosomes that appeared contain acquired during