[22] Precipitation techniques

作者: Sasha Englard , Sam Seifter

DOI: 10.1016/0076-6879(90)82024-V

关键词:

摘要: … is at about 5000 g for 15 min. Most precipitates will sediment rapidly because of the low … In dissolving the precipitate, the buffer is first added in minimum amount, and stirring can be …

参考文章(7)
Malcolm Dixon, Edwin C. Webb, Enzyme Fractionation By Salting-Out: A Theoretical Note Advances in Protein Chemistry. ,vol. 16, pp. 197- 219 ,(1961) , 10.1016/S0065-3233(08)60030-3
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
A.D. McLachlan, Murray Stewart, Tropomyosin coiled-coil interactions: evidence for an unstaggered structure. Journal of Molecular Biology. ,vol. 98, pp. 293- 304 ,(1975) , 10.1016/S0022-2836(75)80119-7
Andrew D. McLachlan, Jonathan Karn, Periodic features in the amino acid sequence of nematode myosin rod Journal of Molecular Biology. ,vol. 164, pp. 605- 626 ,(1983) , 10.1016/0022-2836(83)90053-0
R. Czok, Th. Bücher, Crystallized enzymes from the myogen of rabbit skeletal muscle. Advances in Protein Chemistry. ,vol. 15, pp. 315- 415 ,(1961) , 10.1016/S0065-3233(08)60311-3