Folate status, metabolic genotype, and biomarkers of genotoxicity in healthy subjects

摘要: Gene-environment interactions play an important role in folate metabolism, with a potential impact on human health. Deficiencies the uptake of key micronutrients and variant genotypes can affect folic acid cycle, modulating methyl group transfer processes leading to increased cancer risk Down syndrome incidence. So far, significance status metabolic baseline levels DNA damage normal individuals has not been fully elucidated. In this study, possible modulation SCE, micronuclei tail moment values peripheral lymphocytes by plasma acid, homocysteine vitamin B12, methylenetetrahydrofolate reductase (MTHFR) C677T methionine synthase (MTRR) A66G polymorphisms was investigated 191 healthy subjects. The results obtained show highly significant (P = 0.001) positive association between B12 frequencies both SCE high frequency cells (HFC, above 90 degrees percentile) smokers. No effect observed non-smokers. Moreover, after correction for age, gender GSTM1 genotype, 0.026) MTRR 66GG genotype higher micronucleus rates observed. Tail were affected any independent variables considered. Overall, suggest that relevant influence background smokers may highlight methylation repair, although other, unidentified dietary factors cannot be ruled out. At same time, data indicate represent another individual trait relative genomic instability, thus supporting epidemiological conception