Characterization of glycol chitosan grafted with low molecular weight polyethylenimine as a gene carrier for human adipose-derived mesenchymal stem cells
作者： Yoonhee Bae , Young Hwa Lee , Sunray Lee , Jin Han , Kyung Soo Ko
DOI: 10.1016/J.CARBPOL.2016.07.115
关键词:
摘要: Mesenchymal stem cells (MSCs) have a great capacity for self-renewal while still maintaining their multipotency, and can differentiate into variety of cell types. The delivery genes to site injury is current interesting field gene therapy. In the present study, we describe nonviral carrier, glycol chitosan-methyl acrylate-polyethylenimine (GMP) polymer targeted towards human adipose-derived mesenchymal (AD-MSCs). Transfection efficiency, using luciferase (Luc) pDNA encoding enhanced green fluorescent protein (EGFP), along with cytotoxicity assays, were performed in AD-MSCs. results show that transfection efficiency GMP was similar PEI25kD, lower. Moreover, AD-MSCs treated polymer/pDNA polyplex its cellular uptake distribution analyzed by flow cytometry confocal microscopy. Furthermore, endosomal escape analysis LysoTracker Red, found conjugated could from endosome cytosol. Human maintained potential osteogenic differentiation phenotypic expression based on analysis. study demonstrates be used as targeted-delivery carrier effective delivery.
sci-hub.se 参考文章(38)
Dan Luo, W. Mark Saltzman, Synthetic DNA delivery systems Nature Biotechnology. ,vol. 18, pp. 33- 37 ,(2000) , 10.1038/71889
Young Hwa Lee, Hae In Park, Joon Sig Choi, Novel glycol chitosan-based polymeric gene carrier synthesized by a Michael addition reaction with low molecular weight polyethylenimine. Carbohydrate Polymers. ,vol. 137, pp. 669- 677 ,(2016) , 10.1016/J.CARBPOL.2015.10.089
Yun-Xia Sun, Wang Xiao, Si-Xue Cheng, Xian-Zheng Zhang, Ren-Xi Zhuo, Synthesis of (Dex-HMDI)-g-PEIs as effective and low cytotoxic nonviral gene vectors. Journal of Controlled Release. ,vol. 128, pp. 171- 178 ,(2008) , 10.1016/J.JCONREL.2008.03.004
Herbert H Engelhard, Gene therapy for brain tumors: The fundamentals Surgical Neurology. ,vol. 54, pp. 3- 9 ,(2000) , 10.1016/S0090-3019(00)00234-2
Sania Mansouri, Patrick Lavigne, Karin Corsi, Mohamed Benderdour, Eric Beaumont, Julio C Fernandes, None, Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy European Journal of Pharmaceutics and Biopharmaceutics. ,vol. 57, pp. 1- 8 ,(2004) , 10.1016/S0939-6411(03)00155-3
Hongtao Lv, Shubiao Zhang, Bing Wang, Shaohui Cui, Jie Yan, Toxicity of cationic lipids and cationic polymers in gene delivery. Journal of Controlled Release. ,vol. 114, pp. 100- 109 ,(2006) , 10.1016/J.JCONREL.2006.04.014
T. A. Theodossiou, A. Pantos, I. Tsogas, C. M. Paleos, Guanidinylated dendritic molecular transporters: prospective drug delivery systems and application in cell transfection. ChemMedChem. ,vol. 3, pp. 1635- 1643 ,(2008) , 10.1002/CMDC.200800190
Hu-Lin Jiang, You-Kyoung Kim, Rohidas Arote, Jae-Woon Nah, Myung-Haing Cho, Yun-Jaie Choi, Toshihiro Akaike, Chong-Su Cho, Chitosan-graft-polyethylenimine as a gene carrier. Journal of Controlled Release. ,vol. 117, pp. 273- 280 ,(2007) , 10.1016/J.JCONREL.2006.10.025
Hoi Ki Cheung, Tim Tian Y. Han, Dale M. Marecak, John F. Watkins, Brian G. Amsden, Lauren E. Flynn, Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells. Biomaterials. ,vol. 35, pp. 1914- 1923 ,(2014) , 10.1016/J.BIOMATERIALS.2013.11.067
Joon Sig Choi, Kihoon Nam, Jong-yeun Park, Jung-Bin Kim, Ja-Kyeong Lee, Jong-sang Park, Enhanced transfection efficiency of PAMAM dendrimer by surface modification with L-arginine. Journal of Controlled Release. ,vol. 99, pp. 445- 456 ,(2004) , 10.1016/J.JCONREL.2004.07.027