Insulin-like Growth Factor-Dependent Proliferation and Survival of Triple-Negative Breast Cancer Cells: Implications for Therapy

摘要: Triple-negative breast cancers have a poor prognosis and are not amenable to endocrine- or HER2-targeted therapies. The prevailing view is that targeting the insulin-like growth factor (IGF) signal transduction pathway will be beneficial for triple-negative because their IGF-responsive. present study investigates importance of IGFs in proliferation survival cancer cells. Estrogen progesterone receptors, HER2, type I IGF, insulin receptors were measured by Western transfer analysis. effects IGF-1 on assessed DNA quantitation cell poly (ADP-ribose) polymerase cleavage. effect phosphorylation IGF Akt mitogen-activated protein kinase, was Seven lines identified as models shown express at levels similar those estrogen-responsive known respond IGFs. increased all lines. Proliferation attenuated after reduction receptor expression. Cells higher more sensitive subnanomolar concentrations, but magnitude correlated simply with absolute amount kinase. These results show stimulate promote cells warrant investigation therapeutic target treatment cancer.