Formation of multiple complexes between beta-dystroglycan and dystrophin family products

摘要: Beta-dystroglycan is expressed in a wide variety of tissues and has generally been reported with an Mr 43 kDa, sometimes accompanied 31 kDa protein assumed to be truncated product. This molecule was recently identified as the anomalous beta-dystroglycan various carcinoma cell lines. We produced characterized G5 polyclonal antibody specific that directed against C-terminal portion molecule. provide evidence may vary size properties by studying different Xenopus tissues. Besides normal smooth cardiac muscle sciatic nerve extracts, we found it skeletal brain proteins 38 65 respectively. Glycosylation proteolytic susceptibilities these beta-dystroglycans are analysed compared this work. Crosslinking experiments preparations obtained from muscles gave rise new covalent products 125 (doublet band), or 120 130 140 240 evidence, using similar preparations, immunoprecipitation procedure allows consistent pelleting dystrophin-family isoforms. Skeletal reveals presence two distinct complexes, one dystrophin another which involves alpha-dystrobrevin. Cardiac shown contain three complexes related Dystrophin alpha-dystrobrevin Dp71 were Dp180 Up71 brain. variability relationship between isoforms suggests each – currently known associated could not present complexes.