Thin cell layer technology for induced response and control of rhizogenesis in chrysanthemum

摘要: Chrysanthemum (Dendranthema Xgrandiflora Ramat. Kitamura) stem transverse thin celllayers (tTCLs) were used to obtain defined morphogenic programs with selectedplant growth regulators. A rhizogenic pathway could be manipulated invitro by the application of a single auxin (2,4-D, NAA, IBA or IAA,in increasing order response), addition coconutwater, light darkness playing significant role. The TIBAeliminated capacity all auxins tested, but not that ofcoconut water, while activated charcoal was inhibitory. Theabsence sucrose resulted in limited response. Results clearlyindicate importance auxins, media additives and activationof program chrysanthemum tTCLs. Due their restricted size andmedium-dependant nature, control rhizogenesis and/ororganogenesis (and indeed any plant species) TCLs hasfar-reaching consequences applications floricultural andpharmaceutical sectors. Since factors (exogenously-applied hormones andother growth-stimulating growth-inhibiting substances, light, temperature,humidity other environmental cues) may strictly controlled invitro, TCL technology allows for establishment protocols aimedat flower improvement through genetic engineering, success ofwhich lies its first step i.e. programmable morphogenesis regeneration.