Myeloma Cell Immunoglobulin Expression during in vivo Growth in Diffusion Chambers: Evidence for Repetitive Cycles of Differentiation

摘要: The DNP-binding BALB/c plasmacytoma MOPC-315 undergoes repetitive cycles of differentiation immunoglobulin expression when serially passaged at 10-day intervals in normal syngenic mice. Within 24 hr implantation cells i.p. diffusion chambers there is a preferential loss which: a) secrete IgA paraprotein, b) have large amounts cell surface membrane localized and c) morphologically plasmacytic appearance. In addition, myeloma stem capable forming macroscopic splenic colonies following i.v. injection not only survive preferentially, but they also increase number sooner than any other measured subset. Following the period progressive with immunoglobulin, this followed by an which homogeneous immunoglobulin. These observations demonstrate that marked changes frequency absolute numbers subsets occur hosts. It clear observed during initial 7 to 10 days growth would been detected without property used combination permit sequential recovery all surviving their progeny. We interpret our data as indicating linear maturation progeny, raise possibility variation may fundamental implications for idiotype-specific suppression grafts achieved immunization purified homologous