An array of target-specific screening strains for antibacterial discovery

摘要: As the global threat of drug- and antibiotic-resistant bacteria continues to rise, new strategies are required advance drug discovery process. This work describes construction an array Escherichia coli strains for use in whole-cell screens identify antimicrobial compounds. We used recombination systems from bacteriophages λ P1 engineer each strain low-level expression a single, essential gene product, thus making hypersusceptible specific inhibitors that target. Screening nine parallel against large chemical library permitted identification bacterial growth. example target specificity approach, compounds identified screen MurA were also found block biochemical function when tested vitro.