Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains.

摘要: The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference spatial and temporal changes metabolic activities during biofilm formation. Our study seeks examine metabolome low high biofilm-formers using metabolomic approach. Eight clinical strains with different were chosen for analysis. Bacterial metabolites extracted Bligh Dyer method analyzed by Liquid Chromatography/Quadrupole Time-of-Flight mass spectrometry. data was processed MassHunter Qualitative Analysis Mass Profiler Professional programs. Based on global profiles, presented as two distinctly groups. Interestingly, low-biofilm-formers produced more than high-biofilm-formers. Further analysis performed identify that differed significantly (p-value < 0.005) between biofilm-formers. These include major categories lipids involve prostaglandin folate metabolism. findings suggest formation is complex probably driven bacterium’ endogenous Understanding underlying may enhance our current understanding pathogenesis, extragastric survival transmission infections.