Characterization of an in vitro model of human melanoma invasion based on reconstructed human skin.

摘要: The purpose of this study was to compare the invasive properties normal human cutaneous melanocytes and a melanoma cell line (HBL) in three-dimensional model reconstructed skin. Specifically, we asked what extent pigmentary behaviour both cells is influenced by their interaction with adjacent skin (keratinocytes fibroblasts) basement membrane (BM). In presence BM, within remained basal layer keratinocytes did not pigment spontaneously. When BM removed, were found suprabasally pigmented extensively. No significant invasion into dermis detected or absence BM. HBL showed no ability invade other cells, irrespective However, when added fibroblasts, capacity dermis, Associated noted keratinocyte entry dermis. On own, entered but penetration present. summary, demonstrates clear differences between respect properties. It also allows demonstration interactions provides evidence for synergistic penetrating