Miniature direct electron transfer based sulphite/oxygen enzymatic fuel cells.

摘要: Abstract A direct electron transfer (DET) based sulphite/oxygen biofuel cell is reported that utilises human sulphite oxidase ( h SOx) and Myrothecium verrucaria bilirubin Mv BOx) nanostructured gold electrodes. For bioanode construction, the microelectrodes were further modified with 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester) to which polyethylene imine was covalently attached. SOx adsorbed onto this chemically electrode high surface loading of electroactive enzyme in presence anodic bioelectrocatalytic currents generated an onset potential 0.05 V vs. NHE. The biocathode contained BOx directly deposited nanoparticles for cathodic oxygen reduction starting at 0.71 V Both electrodes integrated a DET-type cell. Power densities 8 1 μW cm −2 achieved 0.15 V 0.45 V voltages, respectively, membrane biodevices under aerobic conditions.