Electro-oxidation of Glycerol from Cooking Palm Oil by Dehydrogenase Enzymes Immobilized on Poly(o- phenylenediamine) Modified Electrodes

摘要: This study reported on the electrochemical oxidations of glycerol, obtained from cooking palm oil hydrolyzed using commercial lipase, by two monoenzyme systems: (1) glycerol dehydrogenase (GDH); and (2) alcohol (ADH) immobilized within Nafion layer surface poly(o-phenylenediamine) (PoPD) modified carbon cloth electrode. Another system studied was a bienzyme ADH aldehyde (AldDH) similarly substrate electrode described earlier. The later aimed for possible multi-step oxidation glycerol. Voltammetric studies over potential ranges -1.5 to 0.1V vs. Ag/AgCl (3 M KCl), have showed that these electrodes displayed mostly quasi-reversible type processes Various parameters also been examined experimental optimum conditions. In general, maximum oxidative current density all at pH 7 with weight lipase 0.1-0.15 g concentration nicotine adenosine dinucleotide (NAD + ) 1-3 mM. findings were expected useful in fabrication anodes biofuel cell oil.