Phosphatase activity of the voltage-sensing phosphatase, VSP, shows graded dependence on the extent of activation of the voltage sensor
作者： Souhei Sakata , Yasushi Okamura
DOI: 10.1113/JPHYSIOL.2013.263640
关键词:
摘要: Key points The voltage-sensing phosphatase (VSP) consists of the voltage sensor and domain. The movement is coupled to activity. To uncover coupling mechanisms between two domains, we made a mutant VSP. Our analyses showed that this moves in steps. Measurements revealed activity associated with both first second step movements. Results suggest VSP shows graded dependence on extent activation sensor. Abstract The cytoplasmic region, activity. However, its still remain unclear. One possible scenario activated only when fully state. Alternatively, enzymatic single proteins could be distinct states sensor, partial lead phosphatase. To distinguish these possibilities, studied zebrafish VSP, where steps as evidenced by charge movements clamp fluorometry. Measurements toward phosphatidylinositol 4,5-bisphosphate are tuning activities, consistent idea magnitude sensor.
sci-hub.se 参考文章(24)
Yoshimichi Murata, Yasushi Okamura, Depolarization activates the phosphoinositide phosphatase Ci-VSP, as detected in Xenopus oocytes coexpressing sensors of PIP2. The Journal of Physiology. ,vol. 583, pp. 875- 889 ,(2007) , 10.1113/JPHYSIOL.2007.134775
Hailin Zhang, Cheng He, Xixin Yan, Tooraj Mirshahi, Diomedes E. Logothetis, Activation of inwardly rectifying K+ channels by distinct PtdIns(4,5)P2 interactions. Nature Cell Biology. ,vol. 1, pp. 183- 188 ,(1999) , 10.1038/11103
Alan L. Goldin, Maintenance of Xenopus laevis and oocyte injection. Methods in Enzymology. ,vol. 207, pp. 266- 279 ,(1992) , 10.1016/0076-6879(92)07017-I
Rona Sadja, Karine Smadja, Noga Alagem, Eitan Reuveny, Coupling Gβγ-Dependent Activation to Channel Opening via Pore Elements in Inwardly Rectifying Potassium Channels Neuron. ,vol. 29, pp. 669- 680 ,(2001) , 10.1016/S0896-6273(01)00242-2
Carlos A. Villalba-Galea, Francesco Miceli, Maurizio Taglialatela, Francisco Bezanilla, Coupling between the voltage-sensing and phosphatase domains of Ci-VSP. The Journal of General Physiology. ,vol. 134, pp. 5- 14 ,(2009) , 10.1085/JGP.200910215
H. Iwasaki, Y. Murata, Y. Kim, Md. I. Hossain, C. A. Worby, J. E. Dixon, T. McCormack, T. Sasaki, Y. Okamura, A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4,5-bisphosphate Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 7970- 7975 ,(2008) , 10.1073/PNAS.0803936105
Susy C Kohout, Maximilian H Ulbrich, Sarah C Bell, Ehud Y Isacoff, Subunit organization and functional transitions in Ci-VSP Nature Structural & Molecular Biology. ,vol. 15, pp. 106- 108 ,(2008) , 10.1038/NSMB1320
Md. Israil Hossain, Hirohide Iwasaki, Yoshifumi Okochi, Mohamed Chahine, Shinichi Higashijima, Kuniaki Nagayama, Yasushi Okamura, Enzyme Domain Affects the Movement of the Voltage Sensor in Ascidian and Zebrafish Voltage-sensing Phosphatases Journal of Biological Chemistry. ,vol. 283, pp. 18248- 18259 ,(2008) , 10.1074/JBC.M706184200
Daniel Minor, L Liu, SC Kohout, Q Xu, S Müller, CR Kimberlin, EY Isacoff, DL Minor, A glutamate switch controls voltage-sensitive phosphatase function. Nature Structural & Molecular Biology. ,vol. 19, pp. 633- 641 ,(2012) , 10.1038/NSMB.2289
S. Sakata, S. Yamaguchi, S. Horie, K. J. Homma, T. Sasaki, Y. Okamura, T. Kurokawa, S. Takasuga, 3′ Phosphatase activity toward phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2] by voltage-sensing phosphatase (VSP) Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 10089- 10094 ,(2012) , 10.1073/PNAS.1203799109