Tomato Lycopene Extract Prevents Lipopolysaccharide-Induced NF-κB Signaling but Worsens Dextran Sulfate Sodium-Induced Colitis in NF-κBEGFP Mice

摘要: Background The impact of tomato lycopene extract (TLE) on intestinal inflammation is currently unknown. We investigated the effect TLE lipopolysaccharide (LPS)-induced innate signaling and experimental colitis. Methodology/principal findings Mice were fed a diet containing 0.5 2% or isoflavone free control (AIN-76). therapeutic efficacy was assessed using dextran sulfate sodium (DSS) exposed mice IL-10(-/-);NF-kappaB(EGFP) mice, representing an acute spontaneous chronic colitis model respectively. A mini-endoscope used to determine extent macroscopic mucosal lesions. Murine splenocytes epithelial cells in vitro LPS-induced NF-kappaB signaling. In vitro, blocked IkappaBalpha degradation, RelA translocation, transcriptional activity MIP-2 mRNA accumulation IEC-18 cells. Moreover, IL-12p40 gene expression dose-dependently inhibited TLE-treated splenocytes. Interestingly, DSS-induced worsened TLE-fed NF-kappaB(EGFP) compared as measured by weight loss, colonoscopic analysis histological scores. contrast, displayed decreased colonic EGFP diet. IL-6, TNFalpha, MCP-1 increased colon TLE-fed, DSS-exposed Additionally, caspase-3 activation TUNEL positive enhanced diet-fed, DSS mice. Conclusions/ significance These results indicate that prevents proinflammatory blocking signaling, but aggravates enhancing cell apoptosis.