The Relevance of External Quality Assessment for Molecular Testing for ALK Positive Non-Small Cell Lung Cancer: Results from Two Pilot Rounds Show Room for Optimization

摘要: Background and Purpose: Molecular profiling should be performed on all advanced non-small cell lung cancer with non-squamous histology to allow treatment selection. Currently, this include EGFR mutation testing for ALK rearrangements. ROS1 is another emerging target. rearrangement status a critical biomarker predict response tyrosine kinase inhibitors such as crizotinib. To promote high quality in cancer, the European Society of Pathology has introduced an external assessment scheme. This article summarizes results first two pilot rounds organized 2012-2013. Materials Methods: Tissue microarray slides consisting cell-lines resection specimens were distributed request routine using IHC or FISH. Participation FISH included interpretation four digital images. Results: Data from 173 different laboratories was obtained. Results demonstrate decreased error rates second round both IHC, although still need performing evident. Error obtained by lower than IHC. The lowest observed Conclusion: There large variety enumeration practices. Based study, recommendations methodology, analysis, result reporting issued. External crucial element improve molecular testing.