Analysis for paraquat by second- and fourth-derivative spectroscopy.

摘要: We describe simple, precise methods, based on second and fourth-derivative spectroscopy, of analysis for paraquat in serum, plasma, or dialysis fluid. Plasma paraquat, after ion-pair extraction dithionite reduction, yields a sharp, stable absorption peak at 396 nm. Derivative spectroscopy confers enhanced sensitivity (detection limit, 5 microgram/L) improved rejection spectroscopic interference by diquat, hemolysis, bilirubin, lipemia. amplitudes are linearly related to dichloride concentration from 10 1000 microgram/L the methods (CV 2.7-5.2% 50 concentration) accurate. Results derivative assays 18 cases poisoning correlate well with those radioimmunoassay (r = 0.999). enables monitoring concentrations plasma poisoned patients during treatment hemoperfusion hemodialysis. A rapid assay developed fluid 2 permits efficacy hemodialysis be routinely assessed.