MpSaci is a widespread gypsy-Ty3 retrotransposon highly represented by non-autonomous copies in the Moniliophthora perniciosa genome.

摘要: Transposons are an important source of genetic variation. The phytopathogen Moniliophthora perniciosa shows high level variability but little is known about the role class I elements in shaping its genome. In this work, we aimed characterization a new gypsy/Ty3 retrotransposon species, named MpSaci, M. These largely variable size, ranging from 4 to 15 kb, and harbor direct long terminal repeats (LTRs) with varying degrees similarity. Approximately, all copies non-autonomous as shifts reading frame stop codons were detected. Only two (MpSaci6 MpSaci9) code for GAG POL proteins that possess functional domains. Conserved domains typically not found retrotransposons detected could potentially impact expression neighbor genes. Solo LTRs several LARDs (large derivative) Unusual containing small sequences or without interruptions similar gag different pol presenting levels similarities identified. Methylation was observed MpSaci reverse transcriptase sequences. Distribution analysis indicates present copy number genomes C-, S- L-biotypes perniciosa. addition, C-biotype isolates originating state Bahia have fragments common Amazon region hybridization profiles related chromosomal groups. RT-PCR reveals gene is constitutively expressed increased at least three-fold nutrient depravation even though no insertion observed. findings point out collaborated and, primarily represented by elements, still might contribute generation most cacao pathogen Brazil.