Molecular Cloning and Pro-apoptotic Activity of ICErelII and ICErelIII, Members of the ICE/CED-3 Family of Cysteine Proteases
作者： Neil A. Munday , John P. Vaillancourt , Ambereen Ali , Francesca J. Casano , Douglas K. Miller
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摘要: Abstract Cysteine proteases related to mammalian interleukin-1β-converting enzyme (ICE) and the nematode cell death abnormal ced-3 gene product have been implicated in effector mechanism of apoptotic death. Two novel members this new family ICE/CED-3-related proteases, designated ICErel-II ICErel-III, were cloned from human monocytic cells. Both highly homologous ICE (52% identical) CED-3 (25% both contained absolutely conserved pentapeptide sequence Gln-Ala-Cys-Arg-Asp containing catalytic cysteine residue. Other structural motifs that comparable with suggest ICErel-III are also synthesized as larger proenzymes which proteolytically processed form heterodimeric active enzymes. Pro-interleukin-1β processing activity could not be detected cells transfected or but pro-domain-less truncated forms capable effectively inducing fibroblast apoptosis. may, therefore, participate proteolytic events culminating
sci-hub.se 参考文章(30)
G. Salvesen, D.J. Pickup, T. Komiyama, C.A. Ray, A.D. Howard, E.P. Peterson, N.A. Thornberry, Inhibition of interleukin-1 beta converting enzyme by the cowpox virus serpin CrmA. An example of cross-class inhibition. Journal of Biological Chemistry. ,vol. 269, pp. 19331- 19337 ,(1994) , 10.1016/S0021-9258(17)32171-3
M. Kozak, Structural features in eukaryotic mRNAs that modulate the initiation of translation. Journal of Biological Chemistry. ,vol. 266, pp. 19867- 19870 ,(1991) , 10.1016/S0021-9258(18)54860-2
L A Casciola-Rosen, D K Miller, G J Anhalt, A Rosen, Specific cleavage of the 70-kDa protein component of the U1 small nuclear ribonucleoprotein is a characteristic biochemical feature of apoptotic cell death. Journal of Biological Chemistry. ,vol. 269, pp. 30757- 30760 ,(1994) , 10.1016/S0021-9258(18)47343-7
T Fernandes-Alnemri, G Litwack, E S Alnemri, CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. Journal of Biological Chemistry. ,vol. 269, pp. 30761- 30764 ,(1994) , 10.1016/S0021-9258(18)47344-9
K Kuida, J. Lippke, G Ku, M. Harding, D. Livingston, M. Su, R. Flavell, Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme Science. ,vol. 267, pp. 2000- 2003 ,(1995) , 10.1126/SCIENCE.7535475
D. Cerretti, C. Kozlosky, B Mosley, N Nelson, K Van Ness, T. Greenstreet, C. March, Kronheim, T Druck, L. Cannizzaro, al. et, Molecular cloning of the interleukin-1 beta converting enzyme Science. ,vol. 256, pp. 97- 100 ,(1992) , 10.1126/SCIENCE.1373520
Israel Schechter, Arieh Berger, On the size of the active site in proteases. I. Papain Biochemical and Biophysical Research Communications. ,vol. 27, pp. 157- 162 ,(1967) , 10.1016/S0006-291X(67)80055-X
V Gagliardini, P. Fernandez, R. Lee, H. Drexler, R. Rotello, M. Fishman, J Yuan, Prevention of vertebrate neuronal death by the crmA gene. Science. ,vol. 263, pp. 826- 828 ,(1994) , 10.1126/SCIENCE.8303301
M. J. Kostura, M. J. Tocci, G. Limjuco, J. Chin, P. Cameron, A. G. Hillman, N. A. Chartrain, J. A. Schmidt, Identification of a monocyte specific pre-interleukin 1 beta convertase activity Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 5227- 5231 ,(1989) , 10.1073/PNAS.86.14.5227
Ping Li, Hamish Allen, Subhashis Banerjee, Simon Franklin, Linda Herzog, Cynthia Johnston, Jack McDowell, Michael Paskind, Laura Rodman, Jochen Salfeld, Elizabeth Towne, Daniel Tracey, Scott Wardwell, Feng-Yi Wei, Winnie Wong, Robert Kamen, Tara Seshadri, Mice deficient in IL-1β-converting enzyme are defective in production of mature IL-1β and resistant to endotoxic shock Cell. ,vol. 80, pp. 401- 411 ,(1995) , 10.1016/0092-8674(95)90490-5