Effects of exogenous interferon on L cells persistently infected with Sendai virus.

摘要: A carrier culture of L cells persistently infected with Sendai virus (steady state) designated as L-Sendaits was established a temperature-sensitive strain the virus. When interferon added to fluids from start cultures at permissive (35° C) or non-permissive temperature (38° C), cell-associated infectivity unaffected 35° C, while it unexpectedly enhanced 38° although titrated after further incubation 32° C for 2 days. The titer increased by subculturing in continuous presence C. effect on paradoxical enhancement shown be dose dependent. were successively subcultured 6 times absence interferon, more than 95 per cent contained detectable amount nucleocapsid (NP) antigen whereas could detected only 30–40 interferon. Only when transferred temperature, distinct hemadsorbing and hemagglutinating activities release particles, measured activity fluids, detected. Cells accumulated polypeptides Accumulation specific RNA about twice much that Larger sized (probably 50S) major species two smaller RNAs both treated untreated cells.