Biocontrol of Rhizoctonia solani via Induction of the Defense Mechanism and Antimicrobial Compounds Produced by Bacillus subtilis SL-44 on Pepper (Capsicum annuum L.).

摘要: Pepper seedling wilt disease is the main cause of crop yield reduction. Biocontrol agents are widely used to control plant diseases caused by pathogenic fungi and activate defense systems. Our preliminary work showed that Bacillus subtilis SL-44 played a significant role in reduction severity on pepper plants. To evaluate biological mechanism B. Rhizoctonia solani, activities related enzymes were detected with different treatment this study. Fluorescence microscopy combined dyes induced large amount active oxygen callose accumulation leaves. The defense-related enzyme improved significantly when treated SL-44, including peroxidase, catalase, superoxide dismutase, polyphenol oxidase, phenylalanine ammonia lyase. activity chitinase β-1,3-glucanase SL-44-treated was also enhanced. Furthermore, expression level pepper-resistance gene CaPIN II increased treatment. Besides, filtrate led death fungus fracturing mycelia leaking cell contents. Surfactin, iturin, fengycin found crude extracts, which could be effective antifungal compounds against R. solani. results suggest not only systemic resistance solani jasmonic acid-dependent signaling pathway but produce inhibit or even damage mycelium growth findings study provide novel guidance protection development.