A rapid radiometric assay for dihydrofolate reductase
作者： Ronald Hayman , Roland McGready , Martin B. Van der Weyden
DOI: 10.1016/0003-2697(78)90696-6
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摘要: Abstract A rapid radiochemical procedure for the measurement of dihydrofolate reductase activity is described. The method employs separation radiolabeled substrate from products reaction by precipitation with acetic acid and zinc sulfate. This permits processing samples climinates time-consumlng column chromatography techniques. Good agreement results obtained when radioactive compared to spectrophotometric assay method.
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sci-hub.se 参考文章(9)
Peter B. Rowe, Paul J. Russel, Dihydrofolate reductase. Studies on the activation of the bovine liver enzyme. Journal of Biological Chemistry. ,vol. 248, pp. 984- 991 ,(1973) , 10.1016/S0021-9258(19)44361-5
M.J. Osborn, F.M. Huennekens, Enzymatic Reduction of Dihydrofolic Acid Journal of Biological Chemistry. ,vol. 233, pp. 969- 974 ,(1958) , 10.1016/S0021-9258(18)64688-5
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
R. L. BLAKLEY, Crystalline Dihydropteroylglutamic Acid Nature. ,vol. 188, pp. 231- 232 ,(1960) , 10.1038/188231A0
DeWayne Roberts, An isotopic assay for dihydrofolate reductase. Biochemistry. ,vol. 5, pp. 3549- 3551 ,(1966) , 10.1021/BI00875A023
Sigmund F. Zakrzewski, Jaakko J. Himberg, Preparation of tritiated dihydrofolate and its use in the assay of dihydrofolate reductase Analytical Biochemistry. ,vol. 40, pp. 336- 344 ,(1971) , 10.1016/0003-2697(71)90392-7
B.L. Hillcoat, P.F. Nixon, R.L. Blakley, Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase Analytical Biochemistry. ,vol. 21, pp. 178- 189 ,(1967) , 10.1016/0003-2697(67)90179-0
Sheldon P. Rothenberg, A rapid radioassay for folic acid reductase and amethopterin Analytical Biochemistry. ,vol. 16, pp. 176- 179 ,(1966) , 10.1016/0003-2697(66)90095-9
Geoffrey P. Tauro, David M. Danks, Peter B. Rowe, Martin B. Van der Weyden, Max A. Schwarz, Vernon L. Collins, Bernard W. Neal, Dihydrofolate reductase deficiency causing megaloblastic anemia in two families. The New England Journal of Medicine. ,vol. 294, pp. 466- 470 ,(1976) , 10.1056/NEJM197602262940903