Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections.

摘要: During early-stage drug development, and metabolite distribution studies are carried out in animal tissues using a range of techniques, particularly whole body autoradiography (WBA). While widely employed, WBA has number limitations, including the following: expensive synthesis radiolabeled drugs analyte specificity identification. only images radiolabel. MALDI MSI been shown previously to be advantageous for imaging metabolites sections. Ion mobility separation (IMS) adds further step experiments; demonstrated here is MALDI-IMS-MS rats dosed at 6 mg/kg IV with an anticancer drug, vinblastine precursor ion m/z 811.4 several product ions 793, 751, 733, 719, 691, 649, 524, 355. The within ventricles brain also depicted. Clearly these data removal int...