Comparative effects of Ni(II) and Cu(II) ions and their combinations on redox potential and hydrogen photoproduction by Rhodobacter sphaeroides.

摘要: Abstract The aim of the present work was study comparative effects Cu(II) and Ni(II) their mixture on growth, redox potential, hydrogen (H 2 ) yield ATPase activity in phototrophic purple bacteria R. sphaeroides MDC6522 from Jermuk mineral spring Armenia. It ascertained, that Cu 2 + Ni have different bacterial specific growth rate: presence 5 μM rate ~ 3.2-fold lower comparison with control (no addition), increased ~ 1.5-fold medium . These changes may be resulted by action ions potential ( E h ). Low concentrations had an enhancing effect drop H production. increase concentration 1 to enhanced stimulatory (72 h growth) ~ 3-fold , whereas ~ 1.2 fold control.  + Ni combinations were differed when used separately. When added together, disappeared, which indicated heavy metal mechanisms. Moreover, N ′-dicyclohexylcarbodiimide-sensitive membrane vesicles has been both ions, but Сu influence feebly marked results suggest interaction between these F O -ATPase. Thus, obtained point out discrimination reveal new regulatory pathways enhance