Expression of CD33, CD38, and HLA-DR on CD34+ human fetal liver progenitors with a high proliferative potential.
作者： MO Muench , J Cupp , J Polakoff , MG Roncarolo
DOI: 10.1182/BLOOD.V83.11.3170.3170
关键词:
摘要: High proliferative-potential colony-forming cells (HPP-CFC) have been identified in the bone marrow of mice and adult humans, characterized as a compartment primitive progenitors possibly including stem cells. In this report we describe human fetal liver (FL) source HPP-CFC. These FL HPP-CFC develop clonal cultures presence granulocyte-macrophage colony-stimulating factor (GM-CSF) interleukin-3 (IL-3) within 3 to 4 weeks. The median frequency tissues between 16 21 weeks gestational age was 1 3,000 total After growth, grew colony size 8.3 x 10(4) cells/colony. Using cell-sorting techniques were shown be predominantly contained CD34+ CD33+ CD38- fraction heterogeneous for HLA-DR expression, no differences proliferative capacities observed HLA-DR+ HLA-DR- CD33-HLA-DR- population, previously suggested contain cells, very rare FL, representing approximately 1.7 10(5) light-density containing almost CFC. Therefore, it is possible that are Phenotypic characterization lin -LDFL showed these also CD13+, Thy-1+, CD45RA-, CD45RO-, CD71-, heterogenoeous c-kit expression. data suggest represent myeloid may include
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