Metabolism of prostaglandin D2 in the monkey.

摘要: [3H7]Prostaglandin D2 was biosynthesized and infused into an unanesthetized monkey. The urinary metabolites were isolated subsequently identified by gas chromatography-mass spectrometry. Two pathways of prostaglandin metabolism resulted in with D (3-hydroxycyclopentanone) F (cyclopentane-1,3-diol) ring structures. major metabolite as 9,20-dihydroxy-11,15-dioxo-2,3-dinorprost-5-en-1-oic acid. Nine other reflecting various combinations beta omega oxidation, 15 dehydrogenation, 13-14 reduction. In greater abundance those which had the structure. structure 9,11,15-trihydroxy-2,3-dinorprosta-5,13-dien-1-oic acid (dinor F2 alpha). identified, including alpha itself. These, for most part, structural counterparts ring. Since many found to be identical alpha, quantitative determinations may not a specific indicator biosynthesis. Likewise, data involving measurement biological effect must re-examined account possible contribution D2, response.