Probing the interaction of lysozyme with ciprofloxacin in the presence of different-sized Ag nano-particles by multispectroscopic techniques and isothermal titration calorimetry
作者： Fatemeh Pasban Ziyarat , Ahmad Asoodeh , Zahra Sharif Barfeh , Maliheh Pirouzi , Jamshidkhan Chamani
DOI: 10.1080/07391102.2013.785919
关键词:
摘要: The binding of ciprofloxacin to lysozyme in the presence three Ag nano-particles varying sizes was for first time investigated by multispectroscopic and isothermal titration calorimetry techniques at pH 7.4. results indicated that quenched fluorescence intensity through a static mechanism but size-II nano-particles, there were two kinds interaction behaviors. between occurred via second type site, whereas some changes occurred. secondary structure lysozyme–ciprofloxacin determined circular dichroism. thermodynamic parameters measured according van’t Hoff equation. enthalpy (ΔH○) entropy (ΔS○) calculated be −49.7 (kJ mol−1) −20.1 (J mol−1 K−1), respectively, w...
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