Development and validation of a mass spectrometric detection method of peginesatide in dried blood spots for sports drug testing

摘要: As recently reported, dried blood spot (DBS) analysis is an advantageous technique for doping control purposes due to the minimal invasive sample collection, simple and economic manner, as well low susceptibility manipulation. Its general applicability sports drug testing arena has been shown analytes of various substance classes, all which comprise exclusively molecular mass compounds. The aim present study was investigate whether DBS applicable also (pegylated) peptides with relevance controls. target analyte, peginesatide (Omontys, Hematide), a approved pegylated erythropoietin-mimetic peptide approximately 45 kDa, tested treatment anaemia in patients renal failure, chosen, prohibited elite its assumed endurance enhancing effects. Therefore, detection method employing developed based on extraction, proteolytic digestion cation-exchange purification followed by liquid chromatography–tandem spectrometry analysis. Eventually, assay validated qualitative proved be specific, sensitive (limit detection, 10 ng/mL) precise (relative standard deviations below 18 %), demonstrating suitability peptides.