Pyochelin isolated from Burkholderia arboris KRICT1 carried by pine wood nematodes exhibits phytotoxicity in pine callus

摘要: Pine wilt disease is a very complex known to be caused by the pine wood nematode, Bursaphelenchus xylophilus , and its accompanying bacteria. The phytotoxin-producing bacteria have been reported involved in development of disease. In this study, we attempted characterise phytotoxins produced Burkholderia arboris KRICT1, which was carried nematode. It phytotoxic compounds seedling assay, ethyl acetate (EtOAc) layer fermentation broth strain displayed activity on callus Pinus densiflora. One active compound isolated from EtOAc repeated Sephadex LH-20 column chromatography preparative TLC. Using mainly mass spectroscopy nuclear magnetic resonance (NMR) spectroscopy, chemical structure determined that pyochelin (a mixture two interconvertible diastereoisomers, I II). Pyochelin exposure reduced viability callus. Compared with phenylacetic acid, phytotoxin Bacillus spp. transmitted B. Japan, showed much stronger phytotoxicity. results suggested might play role wilting process