Improved transcriptional activators and their use in mis‐expression traps in Arabidopsis

摘要: The synthetic transcription factor LhG4 has been used in numerous mis-expression studies plants. We show that the sequence encoding activation domain, derived from Saccharomyces cerevisiae GAL4, contains several cryptic polyadenylation signals Arabidopsis. GAL4-derived was modified according to preferred Arabidopsis codon usage, generating LhG4AtO which faithfully transcribed In protoplasts, achieved maximum transactivation of pOp promoter with 10-fold less input DNA than LhG4. same methods were compare 10 other derivatives carried alternative natural or domains. Lh214 and Lh314, contain domains comprising trimers a core acidic directed threefold more GUS expression 20-fold contrast, when expressed CaMV 35S transgenic plants carrying pOp-GUS reporter, Lh314 yielded transformants substantially lower activities constructs including performed similarly. When incorporated into an enhancer-trapping vector, however, enhancer traps approximately twice frequency LhG4, suggesting offer improved performance weaker signals. To increase number patterns available for studies, we describe population enhancer-trap lines obtained background. can transactivate unlinked pOp-green fluorescent protein (pOp-GFP) reporter pattern predicted by staining activity.