Phylogeny of the replication regions of pPT23A-like plasmids from Pseudomonas syringae.

摘要: It was previously shown that most Pseudomonas syringae strains contain one or more plasmids with cross-hybridizing replication regions and other areas of homology, these were designated the pPT23A-like family. The majority encode genes conferring epiphytic fitness resistance to antibacterial compounds those investigated in this study are essential for pathogenicity increased virulence. phylogeny 14 from five P. pathovars studied by comparing a fragment sequence their repA (encoding replicase replication). In phylogenetic tree obtained, four groups (≤88·8% identity between members) could be identified. first group contained three pv. tomato strains, apii strain out seven ranging 88·8 100%. clustering did not reflect host specialization reported relationships. second two glycinea (95·5% identity), it also included sequenced replicon plasmid phaseolicola. remaining distantly related sequences. Hybridization experiments using different transposable elements described as plasmid-borne syringae, showed gene content highly dissimilar, suggesting occurrence major reorganizations. Additionally, native always correlate harbouring determined analysis extragenic repetitive consensus (ERIC) arbitrarily primed PCR (AP-PCR) products. Collectively, results suggest were, cases, acquired early during evolution.