Characterization and allelic polymorphisms of rhesus macaque (Macaca mulatta) IgG Fc receptor genes.

摘要: Macaque models are invaluable for AIDS research. Indeed, initial development of HIV-1 vaccines relies heavily on simian immunodeficiency virus-infected rhesus macaques. Neutralizing antibodies, a major component anti-HIV protective responses, ultimately interact with Fc receptors phagocytic and natural killer cells to eliminate the pathogen. Despite role that play in there is very limited information available these molecules Therefore, this study, macaque CD32 (FcγRII) CD64 (FcγRI) homologues were genetically characterized. In addition, presence CD16 (FcγRIII), CD32, allelic polymorphisms determined group nine animals. Results from study show predicted structures highly similar their human counterparts. extracellular domains 88–90% 94–95% homologous, respectively. Although all cysteines conserved between two species, exhibits additional N-linked glycosylation sites, whereas lacks three them when compared humans. Five CD64, distinct sequences indentified animals examined, indicating relatively high level polymorphism Fcγ receptors. Together, results validate macaques as vaccine antibody while at same time, underscoring need take into account degree genetic heterogeneity present species designing experimental protocols.