Characterization of a cDNA clone encoding a basic protein, TP2, involved in chromatin condensation during spermiogenesis in the mouse.

摘要: Abstract A cDNA clone encoding a small cysteine and serine-rich basic protein has been isolated from mouse testis library. This encodes the homologue of involved in initial phases condensation chromatin during spermiogenesis rats, TP2, based on similarities sequence carboxyl terminus, composition, molecular weight, electrophoretic mobility. Mouse TP2 can be divided into highly domain comprising about one-third polypeptide chain at terminus much less remaining two-thirds amino terminus. The 5' end mRNA contains two in-phase initiation codons both which may used generating polypeptides differ length Southern blots demonstrate that there is single copy gene genome Northern polyadenylated present high essentially equal levels early late haploid cells, it virtually absent meiotic cells.