Inhibition of NF-κB activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol)

摘要: French maritime pine bark extract (Pycnogenol®) displays a variety of anti-inflammatory effects in vivo. Aim this study was to determine whether human plasma after oral intake Pycnogenol contains sufficient concentrations active principles inhibit key mediators inflammation. Blood samples from seven healthy volunteers were obtained before and five days administration 200 mg per day. Plasma statistically significantly inhibited matrix metalloproteinase 9 (MMP-9) release monocytes NF-κB activation. Thus, we provide evidence that bioavailable exert by inhibition proinflammatory gene expression which is consistent with documented clinical observations. We suggest our ex vivo method suitable substantiate molecular pharmacological mechanisms complex plant extracts more focussed rational way compared vitro studies taking into account the processes absorption metabolism.