Engineered Lignin in Poplar Biomass Facilitates Cu-Catalyzed Alkaline-Oxidative Pretreatment

摘要: Both untransformed poplar and genetically modified “zip-lignin” poplar, in which additional ester bonds were introduced into the lignin backbone, subjected to mild alkaline copper-catalyzed hydrogen peroxide (Cu-AHP) pretreatment. Our hypothesis was that zip-lignin would be removed more easily than during this pretreatment, resulting higher sugar yields following enzymatic hydrolysis. We observed improved glucose xylose hydrolysis for compared both alkaline-only pretreatment (56% yield 67% poplar) Cu-AHP (77% 85% poplar). Compositional analysis, glycome profiling, microscopy all supported notion linkages increase delignification improve yields. Essentially no differences noted m...