Perennial peanut (Arachis glabrata Benth.) contains polyphenol oxidase (PPO) and PPO substrates that can reduce post-harvest proteolysis.

摘要: Background Studies of perennial peanut (Arachis glabrata Benth.) suggest its hay and haylage have greater levels rumen undegraded protein (RUP) than other legume forages such as alfalfa (Medicago sativa L.). Greater RUP can result in more efficient nitrogen utilization by ruminant animals with positive economic environmental effects. We sought to determine whether, like red clover (Trifolium pretense L.), contains polyphenol oxidase (PPO) PPO substrates that might be responsible for increased RUP. Results Perennial extracts contain immunologically detectible high activity (>100 nkatal mg−1 protein). Addition caffeic acid (PPO substrate) depleted endogenous reduced proteolysis 90%. phenolics prepared from leaves either transgenic PPO-expressing or control (non-expressing) showed could reduce >70% a PPO-dependent manner. Two abundant likely are present including caftaric acid. Conclusions Perennial together capable inhibiting post-harvest proteolysis, suggesting possible mechanism this forage. Research related optimizing the system forage crops will applicable peanut. Published 2013. This article is U.S. Government work public domain USA