Analysis of phospho- and phosphonosphingolipids by high-performance liquid chromatography.

摘要: A simple and efficient method for the separation of phosphosphingolipids including phosphonosphingolipids by high-performance liquid chromatography is described. mixture authentic lipids consisting sphingomyelin, ceramide phosphorylethanolamine, 2-aminoethylphosphonate, N-methylaminoethylphosphonate was completely separated using a silica gel (Zorbax SIL) column with acetonitrile-methanol-water 72:40:10 (v/v) as eluting solvent. The elution these sphingolipids monitored directly an ultraviolet spectromonitor at 207 nm. practical limit detection each sphingolipid about 0.2 microgram or 0.3 nmol. Using this method, we found that from one to four different phosphono- and/or in fresh-water shellfish can be routinely identified reproducibly quantified.