Echocardiographic assessment of the right heart in mice.

摘要: Transgenic and toxic models of pulmonary arterial hypertension (PAH) are widely used to study the pathophysiology PAH investigate potential therapies. Given expense time involved in creating animal disease, it is critical that researchers have tools accurately assess phenotypic expression disease. Right ventricular dysfunction major manifestation hypertension. Echocardiography mainstay noninvasive assessment right function rodent has advantage clear translation humans whom same tool used. Published echocardiography protocols murine lacking. In this article, we describe a protocol for assessing RV vascular mouse model with dominant negative BMPRII mutation; however, applicable any diseases affecting vasculature or heart. We provide detailed description preparation, image acquisition hemodynamic calculation stroke volume, cardiac output an estimate artery pressure.