Transfer of growth factor receptor mRNA via exosomes unravels the regenerative effect of mesenchymal stem cells.
作者： Ariela Benigni , Susanna Tomasoni , Lorena Longaretti , Cinzia Rota , Marina Morigi
关键词:
摘要: Bone marrow–mesenchymal stem cells (BM-MSC) ameliorate renal dysfunction and repair tubular damage of acute kidney injury by locally releasing growth factors, including the insulin-like factor-1 (IGF-1). The restricted homing BM-MSC at site led us to investigate a possible gene-based communication mechanism between cells. Human (hBM-MSC) released microparticles exosomes (Exo) enriched in mRNAs. A selected pattern transcripts was detected Exo versus parental expressed IGF-1 receptor (IGF-1R), but not mRNA, while hBM-MSC contained both R- lacking IGF-1R exposed hBM-MSC-derived acquired human transcript that translated corresponding protein. Transfer mRNA from cisplatin-damaged proximal (proximal epithelial cell [PTEC]) increased PTEC proliferation. Coincubation damaged with soluble further enhanced These fi...
elsevier.com
sci-hub.se 参考文章(32)
H.F. Heijnen, W. Stoorvogel, H.J. Geuze, M.J. Kleijmeer, K. Denzer, Exosome: from internal vesicle of the multivesicular body to intercellular signaling device. Journal of Cell Science. ,vol. 113, pp. 3365- 3374 ,(2000) , 10.1242/JCS.113.19.3365
J Ratajczak, K Miekus, M Kucia, J Zhang, R Reca, P Dvorak, M Z Ratajczak, Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery. Leukemia. ,vol. 20, pp. 847- 856 ,(2006) , 10.1038/SJ.LEU.2404132
H.M. Aejaz, A.K. Aleem, N. Parveen, M.N. Khaja, M. Lakshmi Narusu, C.M. Habibullah, Stem Cell Therapy—Present Status Annual Meeting of the Indian Society for Organ Transplantation (ISOT). ,vol. 39, pp. 694- 699 ,(2007) , 10.1016/J.TRANSPROCEED.2007.01.069
Witold Filipowicz, Suvendra N. Bhattacharyya, Nahum Sonenberg, Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nature Reviews Genetics. ,vol. 9, pp. 102- 114 ,(2008) , 10.1038/NRG2290
Benoit Février, Graça Raposo, Exosomes: endosomal-derived vesicles shipping extracellular messages Current Opinion in Cell Biology. ,vol. 16, pp. 415- 421 ,(2004) , 10.1016/J.CEB.2004.06.003
H. K. Salem, C. Thiemermann, Mesenchymal Stromal Cells: Current Understanding and Clinical Status† Stem Cells. ,vol. 28, pp. 585- 596 ,(2009) , 10.1002/STEM.269
Maria Eldh, Karin Ekström, Hadi Valadi, Margareta Sjöstrand, Bob Olsson, Margareta Jernås, Jan Lötvall, Exosomes Communicate Protective Messages during Oxidative Stress; Possible Role of Exosomal Shuttle RNA PLoS ONE. ,vol. 5, pp. e15353- ,(2010) , 10.1371/JOURNAL.PONE.0015353
Vamsi K. Gangaraju, Haifan Lin, MicroRNAs: key regulators of stem cells. Nature Reviews Molecular Cell Biology. ,vol. 10, pp. 116- 125 ,(2009) , 10.1038/NRM2621
Alex Yuan, Erica L. Farber, Ana Lia Rapoport, Desiree Tejada, Roman Deniskin, Novrouz B. Akhmedov, Debora B. Farber, Transfer of microRNAs by embryonic stem cell microvesicles. PLOS ONE. ,vol. 4, ,(2009) , 10.1371/JOURNAL.PONE.0004722
Giovanni Camussi, Maria C. Deregibus, Stefania Bruno, Vincenzo Cantaluppi, Luigi Biancone, Exosomes/microvesicles as a mechanism of cell-to-cell communication Kidney International. ,vol. 78, pp. 838- 848 ,(2010) , 10.1038/KI.2010.278