Inhibition of c-myc expression by phosphorothioate antisense oligonucleotide identifies a critical role for c-myc in the growth of human breast cancer.

摘要: In search of critical genes in the mechanism estrogen action human breast cancer, we previously showed that stimulates transcription c- myc gene estrogen-dependent (MCF-7) cells. We have now examined role estrogen-stimulated growth MCF-7 cells through use a synthetic antisense phosphorothioate oligonucleotide to specifically inhibit expression protein. Estrogen induces 5-fold increase protein within 90 min steroid-deprived cells, as detected by Western blot. Prior exposure 10 µm results up 95% inhibition induced estrogen. Antisense- inhibits cell 75% over 9 days and also exerts cytostatic effect on estrogen-independent MDA-MB-231 which show relatively high, constitutive . Sense- antisense-pS2 oligonucleotides no level or either line. These demonstrate both specific durable effects oligonucleotides. Furthermore, these indicate for cancer support hypothesis loss regulation this may be an important factor progression cancer.