Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis).
作者： G. Borriello , M.G. Lucibelli , E. De Carlo , C. Auriemma , D. Cozza
DOI: 10.1016/J.RVSC.2011.05.009
关键词:
摘要: Two hundred and twenty Escherichia coli isolates from 314 Mediterranean water buffalo calves less than 4 weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of virulence factors LT, ST, Stx1, Stx2, haemolysins, intimin, CNF1, CNF2, CDT-I, CDT-II, CDT-III, CDT-IV, F17-related fimbriae (F17a, F17b, F17c, F17d). The prevalence ETEC, STEC NTEC 1.8%, 6.8% 20.9%, respectively. ETEC all LT-positive ST-negative. Stx intimin-positive, Stx1 (80%) more frequent Stx2 (27%). CNF Hly-positive, CNF2 (83%) CNF1 (22%). Susceptibility assays to 11 antimicrobials displayed high rates resistance (>30%) tested. These data show that most prevalent strains in diarrhoeic NTEC, mostly HlyA-positive, strong associations CNF2/CDT-III CNF2/F17c.
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