作者: Louis C. Doré , Timothy M. Chlon , Christopher D. Brown , Kevin P. White , John D. Crispino
DOI: 10.1182/BLOOD-2011-09-380634
关键词:
摘要: There are many examples of transcription factor families whose members control gene expression profiles diverse cell types. However, the mechanism by which closely related factors occupy distinct regulatory elements and impart lineage specificity is largely undefined. Here we demonstrate on a genome wide scale that hematopoietic GATA GATA-1 GATA-2 bind overlapping sets genes, often at sites, as means to differentially regulate target balance between proliferation differentiation. We also reveal switch, entails chromatin occupancy exchange GATA2 GATA1 in course differentiation, operates more than one-third bound genes. The switch equally likely lead transcriptional activation or repression; general, act oppositely In addition, show genomic regions co-occupied ETS ETS1 strongly enriched for marked H3K4me3 occupied Pol II. Finally, comparing erythroid cells megakaryocytes, find presence motifs major discriminator megakaryocyte versus red specification.