Chromatin occupancy analysis reveals genome-wide GATA factor switching during hematopoiesis

作者: Louis C. Doré , Timothy M. Chlon , Christopher D. Brown , Kevin P. White , John D. Crispino

DOI: 10.1182/BLOOD-2011-09-380634

关键词:

摘要: There are many examples of transcription factor families whose members control gene expression profiles diverse cell types. However, the mechanism by which closely related factors occupy distinct regulatory elements and impart lineage specificity is largely undefined. Here we demonstrate on a genome wide scale that hematopoietic GATA GATA-1 GATA-2 bind overlapping sets genes, often at sites, as means to differentially regulate target balance between proliferation differentiation. We also reveal switch, entails chromatin occupancy exchange GATA2 GATA1 in course differentiation, operates more than one-third bound genes. The switch equally likely lead transcriptional activation or repression; general, act oppositely In addition, show genomic regions co-occupied ETS ETS1 strongly enriched for marked H3K4me3 occupied Pol II. Finally, comparing erythroid cells megakaryocytes, find presence motifs major discriminator megakaryocyte versus red specification.

参考文章(51)
Yuji Yamaguchi, Leonard I. Zon, Steven J. Ackerman, Masayuki Yamamoto, Toshio Suda, Forced GATA-1 Expression in the Murine Myeloid Cell Line M1: Induction of c-Mpl Expression and Megakaryocytic/Erythroid Differentiation Blood. ,vol. 91, pp. 450- 457 ,(1998) , 10.1182/BLOOD.V91.2.450.450_450_457
Paolo Romania, Valentina Lulli, Elvira Pelosi, Mauro Biffoni, Cesare Peschle, Giovanna Marziali, MicroRNA 155 modulates megakaryopoiesis at progenitor and precursor level by targeting Ets-1 and Meis1 transcription factors British Journal of Haematology. ,vol. 143, pp. 570- 580 ,(2008) , 10.1111/J.1365-2141.2008.07382.X
Bradley E. Bernstein, Tarjei S. Mikkelsen, Xiaohui Xie, Michael Kamal, Dana J. Huebert, James Cuff, Ben Fry, Alex Meissner, Marius Wernig, Kathrin Plath, Rudolf Jaenisch, Alexandre Wagschal, Robert Feil, Stuart L. Schreiber, Eric S. Lander, A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells Cell. ,vol. 125, pp. 315- 326 ,(2006) , 10.1016/J.CELL.2006.02.041
Tamara Tripic, Wulan Deng, Yong Cheng, Ying Zhang, Christopher R. Vakoc, Gregory D. Gregory, Ross C. Hardison, Gerd A. Blobel, SCL and associated proteins distinguish active from repressive GATA transcription factor complexes. Blood. ,vol. 113, pp. 2191- 2201 ,(2008) , 10.1182/BLOOD-2008-07-169417
M. A. McDevitt, Y. Fujiwara, R. A. Shivdasani, S. H. Orkin, An upstream, DNase I hypersensitive region of the hematopoietic-expressed transcription factor GATA-1 gene confers developmental specificity in transgenic mice Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 7976- 7981 ,(1997) , 10.1073/PNAS.94.15.7976
Stella T. Chou, Eugene Khandros, L. Charles Bailey, Kim E. Nichols, Christopher R. Vakoc, Yu Yao, Zan Huang, John D. Crispino, Ross C. Hardison, Gerd A. Blobel, Mitchell J. Weiss, Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate Blood. ,vol. 114, pp. 983- 994 ,(2009) , 10.1182/BLOOD-2009-03-207944
Louis C. Doré, John D. Crispino, Transcription factor networks in erythroid cell and megakaryocyte development. Blood. ,vol. 118, pp. 231- 239 ,(2011) , 10.1182/BLOOD-2011-04-285981
Y. Fujiwara, C. P. Browne, K. Cunniff, S. C. Goff, S. H. Orkin, Arrested development of embryonic red cell precursors in mouse embryos lacking transcription factor GATA-1 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 12355- 12358 ,(1996) , 10.1073/PNAS.93.22.12355
Emery H. Bresnick, Hsiang-Ying Lee, Tohru Fujiwara, Kirby D. Johnson, Sunduz Keles, GATA Switches as Developmental Drivers Journal of Biological Chemistry. ,vol. 285, pp. 31087- 31093 ,(2010) , 10.1074/JBC.R110.159079
Atsushi Kubo, Vincent Chen, Marion Kennedy, Elizabeth Zahradka, George Q. Daley, Gordon Keller, The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation Blood. ,vol. 105, pp. 4590- 4597 ,(2005) , 10.1182/BLOOD-2004-10-4137