A generalized stoichiometric model of C3, C2, C2+C4, and C4 photosynthetic metabolism.

摘要: The goal of suppressing photorespiration in crops to maximize assimilation and yield is stimulating considerable interest among researchers looking bioengineer carbon-concentrating mechanisms into C3 plants. However, detailed quantification the biochemical activities bundle sheath lacking. This work presents a general stoichiometric model for C3, C2, C2+C4, C4 (SMA) which energetics, metabolite traffic, different decarboxylating enzymes (NAD-dependent malic enzyme, NADP-dependent or phosphoenolpyruvate carboxykinase) are explicitly included. SMA can be used refine experimental data analysis formulate hypothetical scenarios, coded freely available Microsoft Excel workbook. theoretical underpinnings behaviour analysed with range simulations, including (i) an operational conditions; (ii) manipulating plant; (iii) progressively upregulating C2 shuttle photosynthesis; (iv) cycle (v) processes that hypothesized respond transient environmental inputs. Results quantify functional trade-offs, such as electron transport needed meet ATP/NADPH demand, well inherent subtypes. refines our understanding stoichiometry photosynthesis, paramount importance basic applied research.