Specific and Non-Specific Adhesion in Cancer Cells with Various Metastatic Potentials

摘要: The majority of cancer deaths are caused by metastasis, not the parent tumor. During cells detach from their neighbors in tumor, enter circulation system, and invade other organs. Thus metastasis initiates with decrease adhesion between regaining to new organs during circulation. In this chapter, we first introduce altered tumor cells’ behavior malignant transition focus on alterations cell adhesion. Second, discuss cell-cell vs. cell-matrix interaction context hierarchical changes cell-properties metastasis. Finally, review current state-of-the-art devices single-cell measurement present our results non-specific a glass micro probe, using novel force sensor. Adhesion measurements compared for malignant, metastatic (HCT-8), non-metastatic adenocarcinoma (Caco-2), normal monkey kidney MA104 cells. HCT-8 is about twice that Caco-2, insignificant MA104. We then measured specific homotypic rates Coulter Counter PC3M (highly human prostate cancer), PC3 (less than PC3M), HCT8 lines. found, increasing potential. These suggest, while often reduced facilitate detachment high them can strategically maximize adherence invasion diverse tissues