CasuL: A new lectin isolated from Calliandra surinamensis leaf pinnulae with cytotoxicity to cancer cells, antimicrobial activity and antibiofilm effect

摘要: Abstract This work describes the isolation of a lectin (CasuL) from leaf pinnulae Calliandra surinamensis and evaluation its cytotoxic, antimicrobial antibiofilm properties. Proteins extract were precipitated with ammonium sulphate (60% saturation) submitted to Sephadex G-75 chromatography, which yielded isolated CasuL (purification factor: 113). Native is an acidic protein (pI 5.82) relative molecular mass 48 kDa. also oligomeric composed three subunits spectrometry revealed similarities Sorghum bicolor protein. did not undergo unfolding when heated but changes in conformation hemagglutinating activity detected at basic pH. reduce viability human peripheral blood mononuclear cells was toxic leukemic K562 (IC 50 67.04 ± 5.78 μg/mL) breast cancer T47D : 58.75 ± 2.5 μg/ mL). (6.25–800 μg/mL) only showed bacteriostatic effect able biofilm formation by Staphylococcus saprophyticcus aureus (non-resistant oxacillin-resistant isolates). antifungal against Candida krusei causing alterations cell morphology damage wall. In conclusion, C. leaves contain thermo-stable biotechnological potential as antibiofilm, agent.