Metabolic responses of novel cellulolytic and saccharolytic agricultural soil Bacteria to oxygen

摘要: Cellulose is the most abundant biopolymer in terrestrial ecosystems and degraded by microbial communities soils. However, relatively little known about diversity function of soil prokaryotes that might participate overall degradation this biopolymer. The active cellulolytic saccharolytic Bacteria an agricultural were evaluated 16S rRNA (13)C-based stable isotope probing. Cellulose, cellobiose glucose mineralized under oxic conditions slurries to carbon dioxide. Under anoxic conditions, these substrates converted primarily acetate, butyrate, dioxide, hydrogen traces propionate iso-butyrate; production fermentation end-products was concomitant with apparent reduction iron(III). [(13)C]-cellulose mainly novel family-level taxa Bacteroidetes Chloroflexi, a taxon Planctomycetes, whereas facilitated Kineosporiaceae (Actinobacteria) cluster III Clostridiaceae clusters within Bacteroidetes. Active aerobic sub-communities [(13)C]-cellobiose [(13)C]-glucose treatments dominated Intrasporangiaceae Micrococcaceae I (Firmicutes) prevalent treatments. A very large number (i.e. 28) detected did not closely affiliate families, Archaea any These collective findings suggest that: (i) uncultured involved utilization cellulose products its hydrolysis, (ii) community differed phylogenetically from community, (iii) oxygen availability impacted differentially on activity (iv) different redox guilds (e.g. fermenters iron reducers) compete or interact during aerated