TFCP2 activates beta-catenin/TCF signaling in the progression of pancreatic cancer
作者： Dai Yuedi , Cai Yuankun , Zhao Jiaying , Liu Han , Wang Yueqi
DOI: 10.18632/ONCOTARGET.19741
关键词:
摘要: Aberrant activation of beta-catenin/TCF (T-cell factor) signaling is frequently observed in the pancreatic cancer. However, regulation nuclear transcription machinery remains largely unknown. In this study, TFCP2 (transcriptional factor CP2) expression cancer was detected by qPCR, immunohistochemistry and western blot. Western blot, colony formation assay, migration invasion experiment were performed to investigate effects on growth cells. vivo, mouse metastasis models utilized determine ability. blots used evaluate related protein expression. Luciferase reporter assay explore role signaling. We have shown that up-regulated Over-expression promoted growth, migration, cells, while knocking down inhibited invasion, The mechanism study revealed interacted beta-catenin, enhanced interaction between beta-catenin TCF4, activated Taken together, our demonstrated oncogenic roles cancer, suggested might be a target for treatment
sci-hub.st 参考文章(20)
Scott E Schaus, Rachel Gredler, Devaraja Rajasekaran, Ayesha Siddiq, Devanand Sarkar, Ulla Hansen, Paul B Fisher, Prasanna K Santhekadur, Dong Chen, The transcription factor LSF: a novel oncogene for hepatocellular carcinoma. American Journal of Cancer Research. ,vol. 2, pp. 269- 285 ,(2012)
Sang-Min Jang, Jung-Woong Kim, Chul-Hong Kim, Joo-Hee An, Eun-Jin Kang, Chul Geun Kim, Hyun-Jung Kim, Kyung-Hee Choi, Control of transferrin expression by β-amyloid through the CP2 transcription factor FEBS Journal. ,vol. 277, pp. 4054- 4065 ,(2010) , 10.1111/J.1742-4658.2010.07801.X
Susumu Ohwada, Ken-ichi Tago, Shin-ichi Nagai, Yasuo Morishita, Yoji Murata, Tsutomu Nakamura, Tetsu Akiyama, Shungo Adachi, Michiru Nishita, Junko Hyodo, Hiroshi Shibuya, Inhibition of Wnt signaling by ICAT, a novel β-catenin-interacting protein Genes & Development. ,vol. 14, pp. 1741- 1749 ,(2000) , 10.1101/GAD.14.14.1741
B. K. Yoo, L. Emdad, R. Gredler, C. Fuller, C. I. Dumur, K. H. Jones, C. Jackson-Cook, Z.-z. Su, D. Chen, U. H. Saxena, U. Hansen, P. B. Fisher, D. Sarkar, Transcription factor Late SV40 Factor (LSF) functions as an oncogene in hepatocellular carcinoma Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 8357- 8362 ,(2010) , 10.1073/PNAS.1000374107
Zrinka Pagon, Janet Volker, Geoffrey M. Cooper, Ulla Hansen, Mammalian transcription factor LSF is a target of ERK signaling. Journal of Cellular Biochemistry. ,vol. 89, pp. 733- 746 ,(2003) , 10.1002/JCB.10549
Sun-Joo Lee, Dongwon Choi, Hyangshuk Rhim, Hyo-Jung Choo, Young-Gyu Ko, Chul Guen Kim, Seongman Kang, PHB2 interacts with RNF2 and represses CP2c-stimulated transcription Molecular and Cellular Biochemistry. ,vol. 319, pp. 69- 77 ,(2008) , 10.1007/S11010-008-9878-2
Hans Clevers, Roel Nusse, Wnt/β-catenin signaling and disease. Cell. ,vol. 149, pp. 1192- 1205 ,(2012) , 10.1016/J.CELL.2012.05.012
Xi He, Unwinding a Path to Nuclear β-Catenin Cell. ,vol. 127, pp. 40- 42 ,(2006) , 10.1016/J.CELL.2006.09.016
Masaru Katoh, Molecular cloning and characterization of LZIC, a novel gene encoding ICAT homologous protein with leucine zipper domain. International Journal of Molecular Medicine. ,vol. 8, pp. 611- 615 ,(2001) , 10.3892/IJMM.8.6.611
Ren-Hua Fan, Late SV40 factor: a key mediator of Notch signaling in human hepatocarcinogenesis. World Journal of Gastroenterology. ,vol. 17, pp. 3420- 3430 ,(2011) , 10.3748/WJG.V17.I29.3420